3-Methyl-1-butanol production in Escherichia coli: random mutagenesis and two-phase fermentation
نویسندگان
چکیده
منابع مشابه
Metabolic engineering of Escherichia coli for 1-butanol production.
Compared to ethanol, butanol offers many advantages as a substitute for gasoline because of higher energy content and higher hydrophobicity. Typically, 1-butanol is produced by Clostridium in a mixed-product fermentation. To facilitate strain improvement for specificity and productivity, we engineered a synthetic pathway in Escherichia coli and demonstrated the production of 1-butanol from this...
متن کاملSelf-regulated 1-butanol production in Escherichia coli based on the endogenous fermentative control
BACKGROUND As a natural fermentation product secreted by Clostridium species, bio-based 1-butanol has attracted great attention for its potential as alternative fuel and chemical feedstock. Feasibility of microbial 1-butanol production has also been demonstrated in various recombinant hosts. RESULTS In this work, we constructed a self-regulated 1-butanol production system in Escherichia coli ...
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Bashir Sajo Mienda*, Mohd Shahir Shamsir, Faezah Mohd Salleh and Rosli Md. Illias Bioinformatics Research Group (BIRG), Biosciences and Health Sciences Department, Faculty of Biosciences and Medical Engineering, Universiti Teknologi, Malaysia Department of Bioprocess Engineering, Faculty of Chemical Engineering, Universiti Teknologi Malaysia, 81310 Skudai, Johor, Malaysia Department of Biologic...
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Human granulocyte colony stimulating factor (hG-CSF) induces proliferation and differentiation of granulocyte progenitor cells. This glycoprotein is currently being used for treatment of neutropenia, in patients who have undergone bone marrow transplantation. So far, different researchers have tried to enhance hG-CSF biological activity and stability. In this study, Polymerase Chain Reaction (P...
متن کاملEnhanced Hydrogen Production in Escherichia Coli through Chemical Mutagenesis, Gene Deletion, and Transposon Mutagenesis
Enhanced Hydrogen Production in Escherichia coli Through Chemical Mutagenesis, Gene Deletion, and Transposon Mutagenesis. (May 2010) Andrea Juliana Garzon Sanabria, B.S., Universidad Industrial de Santander at Bucaramanga Chair of Advisory Committee: Dr. Thomas K. Wood We demonstrate that hydrogen production can be increased by random mutagenesis using N-methyl-N ́-nitro-N-nitrosoguanidine (MNNG...
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ژورنال
عنوان ژورنال: Applied Microbiology and Biotechnology
سال: 2010
ISSN: 0175-7598,1432-0614
DOI: 10.1007/s00253-009-2401-1